Journal Article

DKFZ-2023-00461

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Patient-derived xenograft mouse models to investigate tropism to the central nervous system and retina of primary and secondary central nervous system lymphoma.

Isbell, L. K. ; Tschuch, C. ; Doostkam, S. ; Waldeck, S. ; Andrieux, G.DKFZ* ; Shoumariyeh, K.DKFZ* ; Lenhard, D. ; Schaefer, H. E. ; Reinacher, P. C. ; Bartsch, I. ; Pantic, M. ; Vinnakota, J. M. ; Kakkassery, V. ; Schorb, E. ; Scherer, F.DKFZ* ; Frey, A. V. ; Börries, M.DKFZ* ; Illerhaus, G. ; Duyster, J.DKFZ* ; Schueler, J. ; von Bubnoff, N.DKFZ*

2023
Wiley-Blackwell Oxford [u.a.]

This record in other databases:  

Please use a persistent id in citations: doi:10.1111/nan.12899

Abstract: How and why lymphoma cells home to the central nervous system and vitreoretinal compartment in primary diffuse large B-cell lymphoma of the central nervous system remain unknown. Our aim was to create an in vivo model to study lymphoma cell tropism to the central nervous system.We established a patient-derived central nervous system lymphoma xenograft mouse model and characterised xenografts derived from 4 primary and 4 secondary central nervous system lymphoma patients using immunohistochemistry, flow cytometry and nucleic acid sequencing technology. In reimplantation experiments, we analysed dissemination patterns of orthotopic and heterotopic xenografts and performed RNA sequencing of different involved organs to detect differences at the transcriptome level.We found that xenografted primary central nervous system lymphoma cells home to the central nervous system and eye after intrasplenic transplantation, mimicking central nervous system and primary vitreoretinal lymphoma pathology, respectively. Transcriptomic analysis revealed distinct signatures for lymphoma cells in the brain in comparison to the spleen as well as a small overlap of commonly regulated genes in both primary and secondary central nervous system lymphoma.This in vivo tumour model preserves key features of primary and secondary central nervous system lymphoma and can be used to explore critical pathways for the central nervous system and retinal tropism with the goal to find new targets for novel therapeutic approaches.

Keyword(s): CNS tropism ; patient-derived xenograft ; primary and secondary central nervous system lymphoma ; primary vitreoretinal lymphoma

Note: 2023 Apr;49(2):e12899

---

Contributing Institute(s):

1. DKTK Koordinierungsstelle Freiburg (FR01)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)

Appears in the scientific report 2023

---

Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DEAL Wiley ; Ebsco Academic Search ; Essential Science Indicators ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

---