Journal Article

DKFZ-2023-01111

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Molecular diagnostic tools for the World Health Organization (WHO) 2021 classification of gliomas, glioneuronal and neuronal tumors; an EANO guideline.

Sahm, F. (First author)DKFZ* ; Brandner, S. ; Bertero, L. ; Capper, D.DKFZ* ; French, P. J. ; Figarella-Branger, D. ; Giangaspero, F. ; Haberler, C. ; Hegi, M. E. ; Kristensen, B. W. ; Kurian, K. M. ; Preusser, M. ; Tops, B. B. J. ; van den Bent, M. ; Wick, W.DKFZ* ; Reifenberger, G.DKFZ* ; Wesseling, P.

2023
Oxford Univ. Press Oxford

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Please use a persistent id in citations: doi:10.1093/neuonc/noad100

Abstract: In the 5th edition of the WHO CNS tumor classification (CNS5, 2021), multiple molecular characteristics became essential diagnostic criteria for many additional CNS tumor types. For those tumors, an integrated, 'histomolecular' diagnosis is required. A variety of approaches exists for determining the status of the underyling molecular markers. The present guideline focuses on the methods that can be used for assessment of the currently most informative diagnostic and prognostic molecular markers for the diagnosis of gliomas, glioneuronal and neuronal tumors. The main characteristics of the molecular methods are systematically discussed, followed by recommendations and information on available evidence levels for diagnostic measures. The recommendations cover DNA and RNA next-generation-sequencing, methylome profiling, and select assays for single/limited target analysis, including immunohistochemistry. Additionally, because of its importance as a predictive marker in IDH-wildtype glioblastomas, tools for the analysis of MGMT promoter status are covered. A structured overview of the different assays with their characteristics, especially their advantages and limitations, is provided, and requirements for input material and reporting of results are clarified. General aspects of molecular diagnostic testing regarding clinical relevance, accessibility, cost, implementation, regulatory and ethical aspects are discussed as well. Finally, we provide an outlook on new developments in the landscape of molecular testing technologies in neuro-oncology.

Keyword(s): WHO classification ; glioma ; glioneuronal tumors ; molecular classification ; neuronal tumors

Note: #EA:B300# / 2023 Oct 3;25(10):1731-1749

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Contributing Institute(s):

1. KKE Neuropathologie (B300)
2. DKTK HD zentral (HD01)
3. KKE Neuroonkologie (B320)
4. DKTK Koordinierungsstelle Berlin (BE01)
5. DKTK Koordinierungsstelle Essen/Düsseldorf (ED01)

Research Program(s):

1. 312 - Funktionelle und strukturelle Genomforschung (POF4-312) (POF4-312)

Appears in the scientific report 2023

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Database coverage:
; Clarivate Analytics Master Journal List ; Current Contents - Clinical Medicine ; Essential Science Indicators ; IF >= 15 ; JCR ; Nationallizenz ; PubMed Central ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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