Journal Article

DKFZ-2023-02434

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Proteome-wide tagging with an H2O2 biosensor reveals highly localized and dynamic redox microenvironments.

Kritsiligkou, P. (First author)DKFZ* ; Bosch, K. (First author)DKFZ* ; Shen, T.-K.DKFZ* ; Meurer, M.DKFZ* ; Knop, M.DKFZ* ; Dick, T. (Last author)DKFZ*

2023
National Acad. of Sciences Washington, DC

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Please use a persistent id in citations: doi:10.1073/pnas.2314043120

Abstract: Hydrogen peroxide (H2O2) sensing and signaling involves the reversible oxidation of particular thiols on particular proteins to modulate protein function in a dynamic manner. H2O2 can be generated from various intracellular sources, but their identities and relative contributions are often unknown. To identify endogenous 'hotspots' of H2O2 generation on the scale of individual proteins and protein complexes, we generated a yeast library in which the H2O2 sensor HyPer7 was fused to the C-terminus of all protein-coding open reading frames (ORFs). We also generated a control library in which a redox-insensitive mutant of HyPer7 (SypHer7) was fused to all ORFs. Both libraries were screened side-by-side to identify proteins located within H2O2-generating environments. Screening under a variety of different metabolic conditions revealed dynamic changes in H2O2 availability highly specific to individual proteins and protein complexes. These findings suggest that intracellular H2O2 generation is much more localized and functionally differentiated than previously recognized.

Keyword(s): genetically encoded probes ; hydrogen peroxide ; redox regulation ; redox signaling

Note: DKFZ-ZMBH Alliance / #EA:A160#LA:A160# / 2023 Nov 28;120(48):e2314043120

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Contributing Institute(s):

1. A160 Redoxregulation (A160)
2. A260 Zellmorphogenese und Signalübermittlung (A260)

Research Program(s):

1. 311 - Zellbiologie und Tumorbiologie (POF4-311) (POF4-311)

Appears in the scientific report 2023

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; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 10 ; JCR ; National-Konsortium ; PubMed Central ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record

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