Journal Article

DKFZ-2023-02477

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Mouse models of pediatric high-grade gliomas with MYCN amplification reveal intratumoral heterogeneity and lineage signatures.

Schoof, M. ; Godbole, S. ; Albert, T. K. ; Dottermusch, M. ; Walter, C. ; Ballast, A. ; Qin, N.DKFZ* ; Olivera, M. B.DKFZ* ; Göbel, C. ; Neyazi, S. ; Holdhof, D. ; Kresbach, C. ; Peter, L.-S. ; Epplen, G. D. ; Thaden, V. ; Spohn, M. ; Blattner-Johnson, M.DKFZ* ; Modemann, F. ; Mynarek, M. ; Rutkowski, S. ; Sill, M.DKFZ* ; Varghese, J. ; Afflerbach, A.-K. ; Eckhardt, A. ; Münter, D. ; Verma, A. ; Struve, N. ; Jones, D.DKFZ* ; Remke, M.DKFZ* ; Neumann, J. E. ; Kerl, K. ; Schüller, U.

2023
Nature Publishing Group UK [London]

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Please use a persistent id in citations: doi:10.1038/s41467-023-43564-w

Abstract: Pediatric high-grade gliomas of the subclass MYCN (HGG-MYCN) are highly aggressive tumors frequently carrying MYCN amplifications, TP53 mutations, or both alterations. Due to their rarity, such tumors have only recently been identified as a distinct entity, and biological as well as clinical characteristics have not been addressed specifically. To gain insights into tumorigenesis and molecular profiles of these tumors, and to ultimately suggest alternative treatment options, we generated a genetically engineered mouse model by breeding hGFAP-cre::Trp53Fl/Fl::lsl-MYCN mice. All mice developed aggressive forebrain tumors early in their lifetime that mimic human HGG-MYCN regarding histology, DNA methylation, and gene expression. Single-cell RNA sequencing revealed a high intratumoral heterogeneity with neuronal and oligodendroglial lineage signatures. High-throughput drug screening using both mouse and human tumor cells finally indicated high efficacy of Doxorubicin, Irinotecan, and Etoposide as possible therapy options that children with HGG-MYCN might benefit from.

Keyword(s): Humans (MeSH) ; Child (MeSH) ; Mice (MeSH) ; Animals (MeSH) ; N-Myc Proto-Oncogene Protein: genetics (MeSH) ; N-Myc Proto-Oncogene Protein: metabolism (MeSH) ; Neuroblastoma: metabolism (MeSH) ; Disease Models, Animal (MeSH) ; Glioma: genetics (MeSH) ; Mutation (MeSH) ; Gene Amplification (MeSH) ; N-Myc Proto-Oncogene Protein ; MYCN protein, human

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Contributing Institute(s):

1. Pädiatische Gliomforschung (B360)
2. B062 Pädiatrische Neuroonkologie (B062)
3. DKTK HD zentral (HD01)
4. DKTK Koordinierungsstelle Essen/Düsseldorf (ED01)

Research Program(s):

1. 312 - Funktionelle und strukturelle Genomforschung (POF4-312) (POF4-312)

Appears in the scientific report 2023

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