Journal Article

DKFZ-2024-00401

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Recording physiological history of cells with chemical labeling.

Huppertz, M.-C. ; Wilhelm, J. ; Grenier, V. ; Schneider, M. W. ; Falt, T. ; Porzberg, N. ; Hausmann, D.DKFZ* ; Hoffmann, D. C. F.DKFZ* ; Hai, L.DKFZ* ; Tarnawski, M. ; Pino, G. ; Slanchev, K. ; Kolb, I. ; Acuna, C. ; Fenk, L. M. ; Baier, H. ; Hiblot, J. ; Johnsson, K.

2024
American Association for the Advancement of Science Washington, DC

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Please use a persistent id in citations: doi:10.1126/science.adg0812

Abstract: Recordings of the physiological history of cells provide insights into biological processes, yet obtaining such recordings is a challenge. To address this, we introduce a method to record transient cellular events for later analysis. We designed proteins that become labeled in the presence of both a specific cellular activity and a fluorescent substrate. The recording period is set by the presence of the substrate, whereas the cellular activity controls the degree of the labeling. The use of distinguishable substrates enabled the recording of successive periods of activity. We recorded protein-protein interactions, G protein-coupled receptor activation, and increases in intracellular calcium. Recordings of elevated calcium levels allowed selections of cells from heterogeneous populations for transcriptomic analysis and tracking of neuronal activities in flies and zebrafish.

Note: ISSN 1095-9203

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Contributing Institute(s):

1. KKE Neuroonkologie (B320)
2. DKTK HD zentral (HD01)

Research Program(s):

1. 312 - Funktionelle und strukturelle Genomforschung (POF4-312) (POF4-312)

Appears in the scientific report 2024

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Chemical Reactions ; Clarivate Analytics Master Journal List ; Current Contents - Agriculture, Biology and Environmental Sciences ; Current Contents - Life Sciences ; Current Contents - Physical, Chemical and Earth Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 50 ; Index Chemicus ; JCR ; National-Konsortium ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection ; Zoological Record

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