Journal Article

DKFZ-2024-02072

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png DNA methylation-array interlaboratory comparison trial demonstrates highly reproducible paediatric CNS tumour classification across 13 international centres.

Chirica, M. ; Jurmeister, P. ; Teichmann, D. ; Koch, A. ; Perez, E.DKFZ* ; Schmid, S.DKFZ* ; Simon, M. ; Driever, P. H. ; Bodden, C.DKFZ* ; van Tilburg, C. M.DKFZ* ; Hardin, E. C.DKFZ* ; Lavarino, C. ; Hench, J. ; Scheie, D. ; Cryan, J. ; Vicha, A. ; Buttarelli, F. R. ; Michiels, A. ; Haberler, C. ; Barahona, P. ; Tops, B. B. J. ; Jacques, T. ; Stokland, T. ; Witt, O.DKFZ* ; Jones, D. T. W. ; Capper, D.DKFZ*

2024
Wiley-Blackwell Oxford [u.a.]

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Please use a persistent id in citations: doi:10.1111/nan.13010

Abstract: DNA methylation profiling, recently endorsed by the World Health Organisation (WHO) as a pivotal diagnostic tool for brain tumours, most commonly relies on bead arrays. Despite its widespread use, limited data exist on the technical reproducibility and potential cross-institutional differences. The LOGGIC Core BioClinical Data Bank registry conducted a prospective laboratory comparison trial with 12 international laboratories to enhance diagnostic accuracy for paediatric low-grade gliomas, focusing on technical aspects of DNA methylation data generation and profile interpretation under clinical real-time conditions.Four representative low-grade gliomas of distinct histologies were centrally selected, and DNA extraction was performed. Participating laboratories received a DNA aliquot and performed the DNA methylation-based classification and result interpretation without knowledge of tumour histology. Additionally, participants were required to interpret the copy number profile derived from DNA methylation data and conduct DNA sequencing of the BRAF hotspot p.V600 due to its relevance for low-grade gliomas. Results had to be returned within 30 days.High technical reproducibility was observed, with a median pairwise correlation of 0.99 (range 0.94-0.99) between coordinating laboratory and participants. DNA methylation-based tumour classification and copy number profile interpretation were consistent across all centres, and BRAF mutation status was accurately reported for all cases. Eleven out of 12 centres successfully reported their analysis within the 30-day timeframe.Our study demonstrates remarkable concordance in DNA methylation profiling and profile interpretation across 12 international centres. These findings underscore the potential contribution of DNA methylation analysis to the harmonisation of brain tumour diagnostics.

Keyword(s): Humans (MeSH) ; DNA Methylation (MeSH) ; Child (MeSH) ; Reproducibility of Results (MeSH) ; Glioma: genetics (MeSH) ; Glioma: diagnosis (MeSH) ; Glioma: pathology (MeSH) ; Brain Neoplasms: genetics (MeSH) ; Brain Neoplasms: diagnosis (MeSH) ; Brain Neoplasms: pathology (MeSH) ; Male (MeSH) ; Female (MeSH) ; Prospective Studies (MeSH) ; Child, Preschool (MeSH) ; CNS tumour ; DNA methylation ; interlaboratory comparison trial

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Contributing Institute(s):

1. DKTK Koordinierungsstelle Berlin (BE01)
2. Pädiatrische Gliomforschung (B360)
3. KKE Pädiatrische Onkologie (B310)
4. DKTK HD zentral (HD01)

Research Program(s):

1. 312 - Funktionelle und strukturelle Genomforschung (POF4-312) (POF4-312)

Appears in the scientific report 2024

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DEAL Wiley ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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