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| Home > Publications database > Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration. |
| Home > Publications database > Identification and characterization of a set of conserved and new regulators of cytoskeletal organization, cell morphology and migration. |
| Journal Article | DKFZ-2024-02231 |
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2011
Springer
Heidelberg
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Please use a persistent id in citations: doi:10.1186/1741-7007-9-54
Abstract: Cell migration is essential during development and in human disease progression including cancer. Most cell migration studies concentrate on known or predicted components of migration pathways.Here we use data from a genome-wide RNAi morphology screen in Drosophila melanogaster cells together with bioinformatics to identify 26 new regulators of morphology and cytoskeletal organization in human cells. These include genes previously implicated in a wide range of functions, from mental retardation, Down syndrome and Huntington's disease to RNA and DNA-binding genes. We classify these genes into seven groups according to phenotype and identify those that affect cell migration. We further characterize a subset of seven genes, FAM40A, FAM40B, ARC, FMNL3, FNBP3/FBP11, LIMD1 and ZRANB1, each of which has a different effect on cell shape, actin filament distribution and cell migration. Interestingly, in several instances closely related isoforms with a single Drosophila homologue have distinct phenotypes. For example, FAM40B depletion induces cell elongation and tail retraction defects, whereas FAM40A depletion reduces cell spreading.Our results identify multiple regulators of cell migration and cytoskeletal signalling that are highly conserved between Drosophila and humans, and show that closely related paralogues can have very different functions in these processes.
Keyword(s): Actin Cytoskeleton: genetics (MeSH) ; Actins: metabolism (MeSH) ; Animals (MeSH) ; Cell Movement: genetics (MeSH) ; Cell Shape: genetics (MeSH) ; Conserved Sequence: genetics (MeSH) ; Drosophila Proteins: genetics (MeSH) ; Drosophila Proteins: metabolism (MeSH) ; Drosophila melanogaster: cytology (MeSH) ; Genes, Insect: genetics (MeSH) ; Genetic Testing (MeSH) ; HeLa Cells (MeSH) ; Humans (MeSH) ; Mice (MeSH) ; NIH 3T3 Cells (MeSH) ; Phenotype (MeSH) ; Proteins: genetics (MeSH) ; Proteins: metabolism (MeSH) ; RNA, Small Interfering: metabolism (MeSH) ; Wound Healing: genetics (MeSH) ; Actins ; Drosophila Proteins ; Proteins ; RNA, Small Interfering
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