Journal Article

DKFZ-2025-01071

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png DNA demethylating agents suppress preclinical models of synovial sarcoma.

Hasegawa, N. ; Benabdallah, N. S. (First author)DKFZ* ; Smith-Fry, K. ; Li, L. ; McCollum, S. ; Li, J. ; Jones, C. A. ; Wagner, L.DKFZ* ; Dalal, V.DKFZ* ; Golde, V.DKFZ* ; Pejkovska, A.DKFZ* ; Carroll, L. ; Haldar, M. ; Pollack, S. M. ; Lowe, S. W. ; Nielsen, T. O. ; Banito, A. (Last author)DKFZ* ; Jones, K. B.

2025
ASCJ Ann Arbor, Mich.

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Please use a persistent id in citations: doi:10.1172/JCI190855

Abstract: Synovial sarcoma is an aggressive soft tissue cancer driven by the chimeric SS18::SSX fusion oncoprotein, which disrupts chromatin remodeling by combining two antagonistic transcriptional regulators. SS18 participates in BAF complexes that open chromatin, while the SSX genes are cancer-testis antigens that interface with chromatin decorated with monoubiquitinated histone H2A placed by Polycomb repressive complexes (PRCs) activity. Because KDM2B brings PRC to unmethylated CpG islands, it is plausible that methylation directly determines the distribution of SS18::SSX to target loci. Given that synovial sarcoma is also characterized by a peculiarly low DNA hypomethylation profile, we hypothesized that further disturbance of DNA methylation would have a negative impact on synovial sarcoma growth. DNMT1 disruption by CRISPR/Cas9 targeting or pharmacologic inhibition with cytidine analogs 5-aza-2'-deoxycytidine (decitabine) and 5-azacytidine led to decreased genome-wide methylation, redistribution of SS18::SSX, and altered gene expression profiles, most prominently including upregulation of tumor suppressor genes, immune-related genes, and mesenchymal differentiation-related genes. These drugs suppressed growth of synovial sarcoma cell lines and drove cytoreduction in mouse genetic models. DNMT1 inhibitors, already approved for treating myelodysplastic syndromes, warrant further clinical investigation for synovial sarcoma as repurposed, targeted treatments exploiting a vulnerability in the intrinsic biology of this cancer.

Keyword(s): Cancer ; Epigenetics ; Expression profiling ; Genetics ; Oncology

Note: #EA:B380#LA:B380# / 2025 Apr 29;135(13):e190855

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Contributing Institute(s):

1. NWG Weichteilsarkome (B380)

Research Program(s):

1. 312 - Funktionelle und strukturelle Genomforschung (POF4-312) (POF4-312)

Appears in the scientific report 2025

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