Journal Article

DKFZ-2025-01224

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Protocol for sub-mucosal orthotopic injection of organoids into murine colon to study tumor growth and metastasis.

Atatri, S. ; Meyers, M. ; Klein, E. ; Brunner, V. ; Tschurtschenthaler, M.DKFZ* ; Letellier, E.

2025
Cell Press Cambridge, MA

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Please use a persistent id in citations: doi:10.1016/j.xpro.2025.103887

Abstract: Orthotopic mouse models of colorectal cancer (CRC) better recapitulate the physiological processes of tumor development and metastatic dissemination. Here, we provide a protocol for colonoscopy-guided transplantation of organoids into the murine colon. We describe the steps for preparing mouse organoids, equipment, and mice for injections, as well as performing colonoscopy-guided mucosal injections and providing subsequent care. This model can be used to investigate various experimental setups, including survival, metastatic potential, and the effects of treatments. For complete details on the use and execution of this protocol, please refer to Felchle et al.1.

Keyword(s): Cancer ; Health Sciences ; Model Organisms ; Organoids

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Contributing Institute(s):

1. DKTK Koordinierungsstelle München (MU01)

Research Program(s):

1. 899 - ohne Topic (POF4-899) (POF4-899)

Appears in the scientific report 2025

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Database coverage:
; ; Article Processing Charges ; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; DOAJ Seal ; Emerging Sources Citation Index ; Fees ; SCOPUS ; Web of Science Core Collection

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