Book/Journal Article

DKFZ-2025-02905

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Differential Analysis of the Nuclear and the Cytoplasmic RNA Interactomes in Living Cells.

Backlund, M. ; Kulozik, A. E.DKFZ*

2022
Humana Press Totowa, NJ
ISBN: 978-1-0716-1974-2 (print), 978-1-0716-1975-9 (electronic)

Abstract: RNA-binding proteins are key mediators of many of the RNA-regulatory functions throughout the RNA life cycle in the nucleus and in the cytoplasm. The invention and the recent refinement of the RNA-interactome capture technology has now enabled the analysis of the global RNA-interactome in living cells in the nucleus and in the cytoplasm separately. This technology thus allows an unprecedented differential view on the function of RNA-binding proteins in these compartments. Here we describe a method combining nucleo-cytoplasmic fractionation and enhanced RNA-interactome capture (eRIC) for studying RBPs binding to polyadenylated RNAs separately in the cytoplasmic and in the nuclear compartments.

Keyword(s): Cell Nucleus: metabolism (MeSH) ; Cytoplasm: metabolism (MeSH) ; RNA: genetics (MeSH) ; RNA: metabolism (MeSH) ; RNA, Messenger: genetics (MeSH) ; RNA-Binding Proteins: metabolism (MeSH) ; Locked nucleic acid ; Nucleo-cytoplasmic fractionation ; RNA ; RNA Interactome capture ; RNA-binding protein ; RNA, Messenger ; RNA-Binding Proteins ; RNA

Note: #LA:A400#

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Contributing Institute(s):

1. KKE Pädiatrische Leukämie (A400)

Research Program(s):

1. 311 - Zellbiologie und Tumorbiologie (POF4-311) (POF4-311)

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Database coverage:
; SCOPUS

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