Journal Article

DKFZ-2017-00140

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Comparison of the tumor cell secretome and patient sera for an accurate serum-based diagnosis of pancreatic ductal adenocarcinoma.

Mustafa, S. A. (First author)DKFZ* ; Pan, L.DKFZ* ; Marzoq, A.DKFZ* ; Fawaz, M.DKFZ* ; Sander, L. A.DKFZ* ; Rückert, F. ; Schrenk, A. ; Hartl, C. ; Uhler, R. ; Yildirim, A.DKFZ* ; Strobel, O.DKFZ* ; Hackert, T. ; Giese, N.DKFZ* ; Büchler, M. W. ; Hoheisel, J.DKFZ* ; Alhamdani, M. S. S. (Last author)DKFZ*

2017
Impact Journals LLC [S.l.]

This record in other databases:  

Please use a persistent id in citations: doi:10.18632/oncotarget.14449

Abstract: Pancreatic cancer is the currently most lethal malignancy. Toward an accurate diagnosis of the disease in body liquids, we studied the protein composition of the secretomes of 16 primary and established cell lines of pancreatic ductal adenocarcinoma (PDAC). Compared to the secretome of non-tumorous cells, 112 proteins exhibited significantly different abundances. Functionally, the proteins were associated with PDAC features, such as decreased apoptosis, better cell survival and immune cell regulation. The result was compared to profiles obtained from 164 serum samples from two independent cohorts - a training and a test set - of patients with PDAC or chronic pancreatitis and healthy donors. Eight of the 112 secretome proteins exhibited similar variations in their abundance in the serum profile specific for PDAC patients, which was composed of altogether 189 proteins. The 8 markers shared by secretome and serum yielded a 95.1% accuracy of distinguishing PDAC from healthy in a Receiver Operating Characteristic curve analysis, while any number of serum-only markers produced substantially less accurate results. Utility of the identified markers was confirmed by classical enzyme linked immunosorbent assays (ELISAs). The study highlights the value of cell secretome analysis as a means of defining reliable serum biomarkers.

Note: 2017 Feb 14;8(7):11963-11976

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Contributing Institute(s):

1. B070 Funktionelle Genomanalyse (B070)
2. Molekulare Grundlagen Gastrointestinaler Tumoren (G180)

Research Program(s):

1. 312 - Functional and structural genomics (POF3-312) (POF3-312)

Appears in the scientific report 2017

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Database coverage:
; BIOSIS Previews ; IF >= 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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