Journal Article

DKFZ-2017-05986

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Overexpression of minichromosome maintenance protein 10 in medulloblastoma and its clinical implications.

Senfter, D. ; Erkan, E. P. ; Özer, E. ; Jungwirth, G. ; Madlener, S. ; Kool, M.DKFZ* ; Ströbel, T. ; Saydam, N. ; Saydam, O.

2017
Wiley New York, NY

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Please use a persistent id in citations: doi:10.1002/pbc.26670

Abstract: Overexpression of minichromosome maintenance (MCM) proteins 2, 3, and 7 is associated with migration and invasion in medulloblastoma (MB). However, expression profiling of all prereplication complex (pre-RC) has not been addressed in MBs.We performed mRNA expression profiling of a large set of pre-RC elements in cell lines and tumor tissues of MB. RNAi technology was employed for functional studies in MB cell lines.Our data showed that most of the pre-RC components are significantly overexpressed in MB. Among all pre-RC mRNAs, MCM10 showed the highest level of expression (∼500- to 1,000-fold) in MB cell lines and tissues compared to the levels detected in cerebellum. In addition, RNAi silencing of MCM10 caused reduced cell proliferation and cell viability in MB cells.Taken together, our study reveals that the pre-RC is dysregulated in MB. In addition, MCM10, a member of this complex, is significantly overexpressed in MB and is required for tumor cell proliferation.

Keyword(s): MCM10 protein, human ; Minichromosome Maintenance Proteins

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Contributing Institute(s):

1. Pädiatrische Neuroonkologie (B062)

Research Program(s):

1. 312 - Functional and structural genomics (POF3-312) (POF3-312)

Appears in the scientific report 2017

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Database coverage:
; BIOSIS Previews ; Current Contents - Clinical Medicine ; IF < 5 ; JCR ; NCBI Molecular Biology Database ; SCOPUS ; Science Citation Index ; Science Citation Index Expanded ; Thomson Reuters Master Journal List ; Web of Science Core Collection

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