Journal Article

DKFZ-2022-01381

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Cell line authentication: a necessity for reproducible biomedical research.

Souren, N. (First author)DKFZ* ; Fusenig, N.DKFZ* ; Heck, S.DKFZ* ; Dirks, W. G. ; Capes-Davis, A. ; Bianchini, F.DKFZ* ; Plass, C. (Last author)DKFZ*

2022
Wiley Hoboken, NJ [u.a.]

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Please use a persistent id in citations: doi:10.15252/embj.2022111307

Abstract: Immortalized or continuous cell lines are invaluable tools in basic and preclinical research. However, the widespread use of misidentified cell lines is a serious threat to scientific reproducibility. Based on the experiences of mandatory cell line authentication at the International Journal of Cancer (IJC), we provide an overview of the issues pertinent to misidentified cell lines and discuss available solutions. We also summarize the lessons learned, revealing that at least 5% of the human cell lines used in manuscripts considered for peer review are misidentified. About 4% of the considered manuscripts are rejected for severe cell line problems, and most are subsequently published in other journals. In order to diminish such malpractice and its consequences for the scientific record, we postulate that strict multi-layered quality control is essential. Besides journals and publishers, we encourage scientists, research institutions, and funders to take action on the matter and revise their respective policies. Hence, we provide concrete recommendations on introducing regular authentication schemes and staff training, and discuss future steps for enhancing good cell culture practices.

Keyword(s): Cell line cross-contamination ; International Journal of Cancer ; good scientific practice ; misidentified cell lines ; research integrity

Note: #EA:ZZ01#LA:B370# / 2022 Jul 18;41(14):e111307

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Contributing Institute(s):

1. Leistungen an Dritte - Journal of Cancer (ZZ01)
2. Epigenomik (B370)

Research Program(s):

1. 312 - Funktionelle und strukturelle Genomforschung (POF4-312) (POF4-312)

Appears in the scientific report 2022

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; DEAL Wiley ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 10 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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