Journal Article

DKFZ-2022-01579

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Nutritional Calcium Supply Dependent Calcium Balance, Bone Calcification and Calcium Isotope Ratios in Rats.

Rott, J. ; Toepfer, E. T. ; Bartosova, M. ; Kolevica, A. ; Heuser, A. ; Rabe, M. ; Behets, G. ; D'Haese, P. C. ; Eichwald, V.DKFZ* ; Jugold, M.DKFZ* ; Damgov, I. ; Zarogiannis, S. G. ; Shroff, R. ; Eisenhauer, A. ; Schmitt, C. P.

2022
Molecular Diversity Preservation International Basel

Abstract: Serum calcium isotopes (δ44/42Ca) have been suggested as a non-invasive and sensitive Ca balance marker. Quantitative δ44/42Ca changes associated with Ca flux across body compartment barriers relative to the dietary Ca and the correlation of δ44/42CaSerum with bone histology are unknown. We analyzed Ca and δ44/42Ca by mass-spectrometry in rats after two weeks of standard-Ca-diet (0.5%) and after four subsequent weeks of standard- and of low-Ca-diet (0.25%). In animals on a low-Ca-diet net Ca gain was 61 ± 3% and femur Ca content 68 ± 41% of standard-Ca-diet, bone mineralized area per section area was 68 ± 15% compared to standard-Ca-diet. δ44/42Ca was similar in the diets, and decreased in feces and urine and increased in serum in animals on low-Ca-diet. δ44/42CaBone was higher in animals on low-Ca-diet, lower in the diaphysis than the metaphysis and epiphysis, and unaffected by gender. Independent of diet, δ44/42CaBone was similar in the femora and ribs. At the time of sacrifice, δ44/42CaSerum inversely correlated with intestinal Ca uptake and histological bone mineralization markers, but not with Ca content and bone mineral density by µCT. In conclusion, δ44/42CaBone was bone site specific, but mechanical stress and gender independent. Low-Ca-diet induced marked changes in feces, serum and urine δ44/42Ca in growing rats. δ44/42CaSerum inversely correlated with markers of bone mineralization.

Keyword(s): bone mineralization ; calcium ; calcium deficiency ; fractionation ; isotope

Note: 2022 Jul 14;23(14):7796

---

Contributing Institute(s):

1. Core Facility-Kleintierbildgebung (W240)

Research Program(s):

1. 315 - Bildgebung und Radioonkologie (POF4-315) (POF4-315)

Appears in the scientific report 2022

---

Database coverage:
; ; ; Article Processing Charges ; Article Processing Charges ; Clarivate Analytics Master Journal List ; Current Contents - Physical, Chemical and Earth Sciences ; DOAJ Seal ; Ebsco Academic Search ; Essential Science Indicators ; Fees ; IF >= 5 ; JCR ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

---