Journal Article

DKFZ-2024-00882

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Protocol for generating and analyzing organ-on-chip using human and mouse intestinal organoids.

Hensel, I. V. ; Steinhauer, M. ; Fairless, R.DKFZ* ; Resnik-Docampo, M.

2024
Elsevier Amsterdam

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Please use a persistent id in citations: doi:10.1016/j.xpro.2024.103037

Abstract: Organ-on-chip technology is a powerful tool for in vitro modeling. Combining it with organoids overcomes lumen inaccessibility while preserving cellular diversity and function of the intestinal epithelium. Here, we present a protocol for generating and analyzing organ-on-chips using human and mouse intestinal organoids. This protocol covers organoid line establishment, single-cell dissociation, chip preparation, and seeding. It outlines procedures for permeability assays, RNA isolation, staining, and imaging. Additionally, we describe independent stimulation and sampling of the apical and basal side.

Keyword(s): cell Biology ; cell culture ; cell isolation ; cell-based assays ; organoids ; tissue Engineering

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Contributing Institute(s):

1. KKE Neuroonkologie (B320)
2. DKTK HD zentral (HD01)

Research Program(s):

1. 312 - Funktionelle und strukturelle Genomforschung (POF4-312) (POF4-312)

Appears in the scientific report 2024

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Database coverage:
; ; Article Processing Charges ; DOAJ Seal ; Fees ; PubMed Central ; SCOPUS

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