Journal Article

DKFZ-2024-01211

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Ovine papillomavirus type 3 virus-like particle-based tools for diagnosis and detection of infection.

Cacciotto, C. ; Dore, G. M. ; Cubeddu, T. ; Burrai, G. P. ; Anfossi, A. G. ; Antuofermo, E. ; Varoni, M. V. ; Demontis, M. P. ; Zobba, R. ; Pittau, M. ; Müller, M.DKFZ* ; Alberti, A.

2024
Elsevier Amsterdam

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Please use a persistent id in citations: doi:10.1016/j.vaccine.2024.06.001

Abstract: The design of prophylactic and diagnostic tools specific to animal papillomaviruses is hampered by the difficulties of viral in vitro manipulation and by the scarce availability of dedicated biotechnological tools. This paper reports the production of Ovine Papillomavirus 3 (OaPV3)-based virus-like particles (OaPV3-VLPs) in the baculovirus system and their use to investigate host humoral immune response through the establishment of an indirect ELISA test., Polyclonal sera and monoclonal antibodies were generated against OaPV3-VLPs, and their isotype and reactivity were determined. Additionally, antibodies allowed OaPV3 detection in ovine squamous cell carcinoma (SCC) samples by immunohistochemistry. Results encourage the standardization of OaPV3-specific prophylactic and serological diagnostic tools, and open new perspectives for the study of host-viral interaction and SCC development.

Keyword(s): Animal papillomavirus ; Oncovirus ; Serogical diagnosis ; Skin cancer ; Vaccines

Note: 2024 Sep 17;42(22):126033

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Contributing Institute(s):

1. Tumorvirus-spez. Vakzinierungsstrategie (D335)

Research Program(s):

1. 314 - Immunologie und Krebs (POF4-314) (POF4-314)

Appears in the scientific report 2024

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 5 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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