Book/Journal Article

DKFZ-2026-02557

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Analysis of Colony Formation Capacity in Ewing Sarcoma.

Zimmermann, M. (First author)DKFZ* ; Cidre-Aranaz, F.DKFZ* ; Ritter, A. (Last author)DKFZ*

2027
Humana Press Totowa, NJ
ISBN: 978-1-0716-5586-3 (print), 978-1-0716-5587-0 (electronic)

Abstract: Colony formation assays enable an assessment of the cells' ability to grow in colonies under in vitro conditions. Following the seeding and an incubation period, the cell colonies are stained using a crystal violet staining solution, and the resulting colonies are imaged and assessed. This method can be leveraged to investigate the effect of genetic modifications or the application of different drug compounds on colony growth. Here, we describe a protocol applied to Ewing sarcoma cell lines, as well as possible modifications.

Keyword(s): Sarcoma, Ewing: pathology (MeSH) ; Humans (MeSH) ; Cell Line, Tumor (MeSH) ; Cell Proliferation (MeSH) ; Cell Culture Techniques: methods (MeSH) ; Colony-Forming Units Assay: methods (MeSH) ; Bone Neoplasms: pathology (MeSH) ; Cell culture ; Clonogenicity ; Colony formation ; Crystal violet ; Ewing sarcoma ; Functional characterization ; In vitro assay ; Long-term proliferation ; Tumorigenicity

Note: #EA:B410#LA:B410# / #NCTZFB26# / First Online: 16 October 2026

---

Contributing Institute(s):

1. Translationale Pädiatrische Sarkomforschung (B410)
2. Koordinierungsstelle NCT Heidelberg (HD02)

Research Program(s):

1. 312 - Funktionelle und strukturelle Genomforschung (POF4-312) (POF4-312)

Appears in the scientific report 2026

---

Database coverage:
; SCOPUS

---