Book/Journal Article

DKFZ-2026-02560

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Proliferation Assessment by Trypan Blue Exclusion in Ewing Sarcoma.

Sauerteig, C. ; Michels, Z. ; Kuehnle, M. ; Funk, C. M. ; Musa, J. (Last author)DKFZ*

2027
Humana Press Totowa, NJ
ISBN: 978-1-0716-5586-3 (print), 978-1-0716-5587-0 (electronic)

Abstract: Cell proliferation is broadly defined as the process that increases cell number, essentially depending on a balance among cell cycle progression, cell division, cell death, and cellular senescence. Deregulation of cell proliferation is a key feature of cancer cells, making assessment of proliferation a central methodological issue in cancer research. Especially in Ewing sarcoma (EwS), which exhibits a high proliferative capacity, experimental assessment of proliferation in preclinical research plays an important role. Among the available methods, Trypan blue exclusion is described here as a robust, easy-to-perform, and cost-effective method for assessing cell proliferation in an experimental setting.

Keyword(s): Humans (MeSH) ; Sarcoma, Ewing: pathology (MeSH) ; Trypan Blue: chemistry (MeSH) ; Cell Proliferation (MeSH) ; Cell Line, Tumor (MeSH) ; Cellular Senescence (MeSH) ; Staining and Labeling: methods (MeSH) ; Cell death ; Cell division ; Ewing sarcoma ; Proliferation ; Senescence ; Trypan blue ; Trypan Blue

Note: #LA:B410#

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Contributing Institute(s):

1. Translationale Pädiatrische Sarkomforschung (B410)

Research Program(s):

1. 312 - Funktionelle und strukturelle Genomforschung (POF4-312) (POF4-312)

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Database coverage:
; SCOPUS

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