Cidre-Aranaz, F. (Editor) ; G. P. Grünewald, T. (Editor) ; Surdez, D. ; Landuzzi, L. ; Scotlandi, K. ; Manara, M. C.

2021
Springer US New York, NY
ISBN: 978-1-0716-1019-0 (print), 978-1-0716-1020-6 (electronic)

2021Ewing Sarcoma / Cidre-Aranaz, Florencia (Editor) ; New York, NY : Springer US, 2021, Chapter 18 ; ISSN: 1064-3745=1940-6029 ; ISBN: 978-1-0716-1019-0=978-1-0716-1020-6 ; doi:10.1007/978-1-0716-1020-6 New York, NY : Springer US, Methods in Molecular Biology 2226, 223 - 242 (2021) [10.1007/978-1-0716-1020-6_18]2021  

Abstract: Ewing sarcoma (EWS) is a rare malignant pediatric tumor and patient derived xenografts (PDXs) could represent a possibility to increase the number of available models to study this disease. Compared to cell derived xenografts (CDX), PDXs are reported to better recapitulate tumor microenvironment, heterogeneity, genetic and epigenetic features and are considered reliable models for their better predictive value when comparing preclinical efficacy and treatment response in patients. In this chapter, we extensively describe a method for generating Ewing sarcoma PDX models, for their validation and molecular characterization.