Journal Article

DKFZ-2026-00335

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Colonic spatial single-cell proteomics and murine models link mitochondrial dysfunction to dimeric IgA-secreting plasma cell deficiency in Crohn's disease.

Raschdorf, A. ; de Almeida, L. N. ; Solbach, P. ; Kirstein, M. M. ; Marquardt, J. U. ; Schmelter, F. ; Günther, U. L. ; Schlichting, H. ; Hicken, M. ; Christiansen, L. ; Wiestler, M. ; Tews, H. C. ; Bettenworth, D. ; consortium, O.-C. (Collaboration Author) ; Sültmann, H.DKFZ* ; Peipp, M. ; Valerius, T. ; Ragab, M. ; Sauer, T. ; Gemoll, T. ; Ehlers, M. ; Rosenstiel, P. ; Manz, R. A. ; Künstner, A. ; Busch, H. ; Sina, C. ; Derer, S. ; Katalinic, A. (Contributor) ; Oberländer, M. (Contributor) ; Deck, R. (Contributor) ; Sültmann, H. (Contributor)DKFZ* ; von Bubnoff, N. (Contributor) ; Hutzenlaub, T. (Contributor) ; Jülg, P. (Contributor) ; Burziwoda, D. (Contributor)

2026
Springer Nature [London]

Abstract: Secretory IgA (SIgA) is critical for maintaining the intestinal barrier. A dysregulated B-cell compartment and altered Ig secretion have been well documented in Crohn's disease (CD) patients, although their origin is unknown. To unravel the role of mucosal humoral immunity in CD pathogenesis, we in-depth phenotype colonic plasma cell (PC) differentiation in CD at the single-cell level, linked to ex vivo functional characterization and experimental mouse models with a congenital mitochondrial defect or under glucose-free high-protein dietary intervention. Here, we demonstrate that despite expanded colonic B cells, CD patients in remission present significantly diminished mucosal dimeric IgA and fecal SIgA. Colonic plasmablasts and immature CD19+CD45+ PCs are increased at the expense of the mature CD19-CD45- phenotype. Accordingly, CD-derived ex vivo differentiated PCs display impaired maturation into dimeric IgA-secreting PCs. In this study, patient-derived data from colonic RNA-seq, spatial single-cell proteomics, and plasma metabolomics are combined with data from both mouse models and highlight the crucial role of mitochondrial oxidative phosphorylation in colonic IgA+-PC differentiation, suggesting promising directions for future therapeutic strategies.

Keyword(s): Animals (MeSH) ; Crohn Disease: immunology (MeSH) ; Crohn Disease: metabolism (MeSH) ; Crohn Disease: pathology (MeSH) ; Plasma Cells: metabolism (MeSH) ; Plasma Cells: immunology (MeSH) ; Plasma Cells: pathology (MeSH) ; Colon: immunology (MeSH) ; Colon: pathology (MeSH) ; Colon: metabolism (MeSH) ; Mice (MeSH) ; Mitochondria: metabolism (MeSH) ; Mitochondria: pathology (MeSH) ; Disease Models, Animal (MeSH) ; Humans (MeSH) ; Proteomics: methods (MeSH) ; Single-Cell Analysis (MeSH) ; Immunoglobulin A, Secretory: metabolism (MeSH) ; Immunoglobulin A, Secretory: immunology (MeSH) ; Male (MeSH) ; Female (MeSH) ; Intestinal Mucosa: immunology (MeSH) ; Intestinal Mucosa: metabolism (MeSH) ; Intestinal Mucosa: pathology (MeSH) ; Mice, Inbred C57BL (MeSH) ; Cell Differentiation (MeSH) ; B-Lymphocytes: immunology (MeSH) ; B-Lymphocytes: metabolism (MeSH) ; Oxidative Phosphorylation (MeSH) ; Immunoglobulin A, Secretory

Note: #NCTZFB26#

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Contributing Institute(s):

1. B063 Krebsgenomforschung (B063)
2. Koordinierungsstelle NCT Heidelberg (HD02)

Research Program(s):

1. 312 - Funktionelle und strukturelle Genomforschung (POF4-312) (POF4-312)

Appears in the scientific report 2026

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