Journal Article

DKFZ-2024-01706

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Affinity-independent memory B cell origin of the early antibody-secreting cell response in naive individuals upon SARS-CoV-2 vaccination.

Li, Z. (First author)DKFZ* ; Obraztsova, A. (First author)DKFZ* ; Shang, F.DKFZ* ; Oludada, O.DKFZ* ; Malapit, J.DKFZ* ; Busch, K.DKFZ* ; van Straaten, M.DKFZ* ; Stebbins, E.DKFZ* ; Murugan, R.DKFZ* ; Wardemann, H. (Last author)DKFZ*

2024
Elsevier New York, NY

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Please use a persistent id in citations: doi:10.1016/j.immuni.2024.07.023

Abstract: Memory B cells (MBCs) formed over the individual's lifetime constitute nearly half of the circulating B cell repertoire in humans. These pre-existing MBCs dominate recall responses to their cognate antigens, but how they respond to recognition of novel antigens is not well understood. Here, we tracked the origin and followed the differentiation paths of MBCs in the early anti-spike (S) response to mRNA vaccination in SARS-CoV-2-naive individuals on single-cell and monoclonal antibody levels. Pre-existing, highly mutated MBCs showed no signs of germinal center re-entry and rapidly developed into mature antibody-secreting cells (ASCs). By contrast, and despite similar levels of S reactivity, naive B cells showed strong signs of antibody affinity maturation before differentiating into MBCs and ASCs. Thus, pre-existing human MBCs differentiate into ASCs in response to novel antigens, but the quality of the humoral and cellular anti-S response improved through the clonal selection and affinity maturation of naive precursors.

Keyword(s): SARS-CoV-2 ; affinity maturation ; germinal center ; humoral immunity ; mRNA vaccination ; pre-existing memory B cells ; somatic hypermutation

Note: #EA:D130#LA:D130# / 2024 Sep 10;57(9):2191-2201.e5

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Contributing Institute(s):

1. Zelluläre Immunologie (D110)
2. Strukturbiologie von Infektion und Immunität (D160)
3. B-Zell-Immunologie (D130)

Research Program(s):

1. 314 - Immunologie und Krebs (POF4-314) (POF4-314)

Appears in the scientific report 2024

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Database coverage:
; BIOSIS Previews ; Biological Abstracts ; Clarivate Analytics Master Journal List ; Current Contents - Life Sciences ; Ebsco Academic Search ; Essential Science Indicators ; IF >= 30 ; JCR ; Nationallizenz ; SCOPUS ; Science Citation Index Expanded ; Web of Science Core Collection

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