Journal Article

DKFZ-2025-02253

http://join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Preanalytical framework for routine clinical use of liquid biopsies: combining EVs and cfDNA

Simon, N. (First author)DKFZ* ; Volz, S.DKFZ* ; Rios de los Rios Reséndiz, J.DKFZ* ; Wedig, T.DKFZ* ; Montigel, S. H.DKFZ* ; Schwarz, N.DKFZ* ; Richter, K.DKFZ* ; Helm, D.DKFZ* ; Neßling, M.DKFZ* ; Zielske, L.DKFZ* ; Berker, J.DKFZ* ; Russeck, S.DKFZ* ; Mauermann, M.DKFZ* ; Hofmann, W.-K. ; Pfister, S. M.DKFZ* ; Pajtler, K. W.DKFZ* ; Maaß, K. K.DKFZ* ; Clemm von Hohenberg, K. (Last author)DKFZ*

2025

Abstract: Aim: Liquid biopsies hold significant potential for the minimally invasive diagnosis of tumors and other diseases.While the clinical application of cell-free DNA (cfDNA) methodologies is emerging, the implementation of tumor-derived extracellular vesicles (EVs) as validated biomarkers is hindered by substantial preanalytical variations. In this work, we standardized the preanalytical procedures of blood collection for subsequent serial isolation of plasma cfDNA and EVs from a single blood collection tube.

Note: Missing Journal: Extracellular Vesicles and Circulating Nucleic Acids (Extracell Vesicles Circ Nucleic Acids.) = 2767-6641 (import from CrossRef, Journals: inrepo02.dkfz.de) / #EA:B062#LA:B470# / epub

---

Contributing Institute(s):

1. B062 Pädiatrische Neuroonkologie (B062)
2. DKTK HD zentral (HD01)
3. Translationale Lymphomforschung (B470)
4. Elektronenmikroskopie (W230)
5. Proteomics (W120)

Research Program(s):

1. 312 - Funktionelle und strukturelle Genomforschung (POF4-312) (POF4-312)

---